Skip to main content
SpringerLink
Log in

Plasmid-Derived Cloning Vectors

  • Protocol
Molecular Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

  • 1585 Accesses

Abstract

Plasmids, circular molecules of DNA that rephcate autonomously in a bacterial host cell, are widely distributed among prokaryotes They vary in size from a few to several hundred ktlobase pairs Although generally not essential to cellular survival, plasmids can confer a selecttve advantage on then host by encoding phenotypes, such as resistance to antibiotics, heavy-metal resistance, production of toxms or other virulence factors, bacteriocm production, or the catabolism of aromatic compounds Naturally occurring plasmids often have characteristics that make them ideally suited for development as clomng vectors Particularly important in this respect has been the exploitation of the many and varied anttbiotic-resistance mechanisms encoded by plasmids 1,2

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Cohen, S N and Mrller, C A (1969) Multrple molecular specres of circular n-factor DNA Isolated from Escherzchza coli Nature 224, 1273–1277

    CAS  Google Scholar 

  2. Russell, A D and Chopra, I (1996) Understanding Antibacterial Action and Resistance Horwood, London, UK

    Google Scholar 

  3. Thomas, C in (1989) Promzscuous Plasmzds of Gram-Negatzve Bacterza Academic, UK

    Google Scholar 

  4. Bohvar, F, Rodriguez, R L, Betlach, in C, and Boyer, H W (1977) Constructron and characterrsatron of new cloning vehicles I Amplcrlhn resistant derrvatrves of the plasmidpMB9 Gene 2,75–93

    Article  Google Scholar 

  5. Bohvar, F, Rodriguez, R L, Greene, P J, Betlach, M C, Heyneker, H L, Boyer, H W, Crosa, J H, and Falkow, S (1977) Construction and characterlsatton of new cloning vectors II A multipurpose cloning system Gene 2,95–113

    Article  Google Scholar 

  6. Sutchffe, G (1979) Complete nucleotlde sequence of the Escherzchla coli plasmld pBR322 Cold Spring Harbor Symp Quant biol 43,77–90

    Google Scholar 

  7. Bohvar, F (1978) Construction and characterlsation of new cloning vehicles III Denvatlves of plasmld pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules Gene 4, 121–136

    Article  Google Scholar 

  8. Vlelra, J and Messing, J (1982) The pUC plasmlds an M13mp7-derived system for msertion mutagenesis and sequencing with synthetic universal primers Gene 19,259–268

    Article  Google Scholar 

  9. Stevls, P E and Ho, N W Y (1987) Positive selec,tion vectors based on xylose ultlhsationsupresslon Gene 55,67–74

    Article  Google Scholar 

  10. Stoker, N G, Fanweather, N F, and Syratt, B G (1982) Versatile low-copy-number plasmld vectors for clomng in Escherlchza co11 Gene 18,335–341

    CAS  Google Scholar 

  11. Uhlm, B E, Schwelckart, V., and Clark, A. J (1983) New runaway-replication-plasmld cloning vectors and suppression of runaway repllcation by novoblocm Gene 22, 255–265

    Article  Google Scholar 

  12. Bagdasanan, M, Franklin, F C H, Lurz, R, Ruckert, B, Elagdasanan, M in, and Tlmmls, K N (1981) Specific purpose cloning vectors II Broad host range, high copy number RSF 10 IO-derived vectors and a hostvector system for gene cloning in Pseudomonas Gene 16,237–247

    Article  Google Scholar 

  13. Blatny, J in, Brautaset, T, Wmther-Larsen, H C, Haugan, K and Valla, S (1997) Construction and use of a versatile set of broad-host-range clomng and expression vectors based on the RK2 rephcon Appl Environ Mwrobiol 63, 370–379

    CAS  Google Scholar 

  14. DeShazer, D and Woods, D E (1996) Broad-host-range cloning and cassette vectors based on the R388 trimethoprlm resistance gene BioTechniques 20,762–764

    PubMed  CAS  Google Scholar 

  15. Ehrhch, S D (1977) Rephcation and expression of plasmlds from Staphylococcus aureus in Bacillus subtills Proc Natl Acad Sci USA 74, 1680–1682

    Article  Google Scholar 

  16. Halma, P, Bron, S, and Venema, G (1987) The effect of restrlction on shotgun cloning and plasmld stability in Bacdlus subtdls Marburg Mol Gen Genet 209, 335–342

    Article  Google Scholar 

  17. Yelton, M in, Hamer, J E, and Timberlake, W E (1984) Transformation of Asperg& lus nldulans by using a trpc plasmld. Proc Natl Acad Sci USA 81, 1470–1474

    Article  PubMed  CAS  Google Scholar 

  18. Leemans, J, Langenakens, J, de Greve, H, Deblaere, R, van Montagu, M, and Schell, J (1982) Broad host range cloning vectors derived from the W plasmld SaGene 19, 361–364

    Article  PubMed  CAS  Google Scholar 

  19. Murray, J A H (1987) Bending the rules the 2~ plasmld of yeast Mol Microbiol 1, 114

    Article  Google Scholar 

  20. Beggs, J D (1978) Transformation of yeast by a replicating hybrid plasmld Nature 275,104–109

    Article  PubMed  CAS  Google Scholar 

  21. Broach, J R (1983) Construction of high copy yeast vectors using 2-micron circle sequences Methods Eniymol 101,307–325

    CAS  Google Scholar 

  22. Chen, X J, Wesolowskl-Louvel, M, Tanguy-Rougeau, C, Blanchi, M in, Fablam, L, Sahola, M, Falcone, C, Frontah L and Fukuhara, H (1988) A gene-cloning system for KZuyreromyces la&s and lsolation of a chromosomal gc ne required for killer toxin production J Basic Mtcrobiol 28,211–220

    Article  CAS  Google Scholar 

  23. Gunge, N and Sakaguchl, K (1981) IntergenerIc transfer of deoxyrlbonuclelc acid killer plasmlds pGKL 1 and pGKL2 from Kluyveromyces lactls into Saccharomyces cerevwae by cell fusion J Bacterlol 145,382–390

    CAS  Google Scholar 

  24. Mead, D A, Knsty Pey, N, Hermstadt, C, Marcll, R H, and Simth, L in (1991) A umversal method for the direct cloning of PCR amplified nucleic acid Bio/Technology 9,657–663

    Article  PubMed  CAS  Google Scholar 

  25. Stanley, K K and Luzlo, J P (1984) Construction of a new family of high efficiency bacterial expression vectors ldentlfication of cDNA clones coding for human liver proteins EMBO J 3, 1429–1434

    PubMed  CAS  Google Scholar 

  26. Remaut, E, Stanssens, P, and Flers, W (1981) Plasmld vectors for high-efficiency expression controlled by the pL promoter of cohphage lambda Gene 15, 81–83

    Article  PubMed  CAS  Google Scholar 

  27. Stark, M J R (1987) Multicopy expresslon vectors carrying the Zuc repressor gene for regulated high-level expression of genes in Escherzchza co11Gene 51,255–267

    Article  PubMed  CAS  Google Scholar 

  28. Melton, D A, Kneg, P A, Rebaghatl, M R, Mamatts, T, Zmn, K, and Green, M R (1984) Efficient in vitro synthesis of biologlcally active RNA and DNA hybrldlsation probes from plasmlds containing bacteriophage SP6 promoter Nucleic Aczds Res 12, 7035–7056

    Article  CAS  Google Scholar 

  29. Lusky, M and Botshan, M (1981) Inhlbltion of SV40 rephcation in simian cells by specific pBR322 sequences Nature 293,79–81

    Article  PubMed  CAS  Google Scholar 

  30. Jlmmez, A and Davies, J (1980) Expresston of a transposable antlblotlc resistance element in Saccharomyces Nature 287,869–871

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Btomedtcal Sciences, Bradford Untversity, Bradford, UK

    Craig Winstanley

  2. University of Hertfordshire, Hatfield, UK

    Ralph Rapley

Authors
  1. Craig Winstanley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ralph Rapley
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Herfordshire, Hatfield, UK

    Ralph Rapley  & John M. Walker  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc , Totowa, NJ

About this protocol

Cite this protocol

Winstanley, C., Rapley, R. (1998). Plasmid-Derived Cloning Vectors. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-59259-642-3_16

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-501-0

  • Online ISBN: 978-1-59259-642-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation