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Synthesis of [15N]-Labeled DNA Fragments

  • Protocol
Protocols for Oligonucleotide Conjugates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 26))

Abstract

DNA fragments labeled with 15N have the potential to provide novel insight into base pairing, hydration, drug/nucleic acid, and protein/ nucleic acid interactions. Synthetic routes to a variety of [15N]-labeled pyrimidine nucleosides (1–3), purines (4–6), and purine nucleosides (7–13) have been reported. In some cases these labeled bases or monomers have been incorporated into nucleic acids, either by chemical synthesis (9,10,14–17) or by biosynthetic procedures (18–22). The focus of this chapter will be on the preparation of [15N]-labeled purine 2′-deoxynucleosides and their incorporation into DNA fragments by chemical synthesis.

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Authors and Affiliations

  1. Department of Chemistr, Rutgers, The State Universit of New Jersey, Piscatatway, NJ

    Roger A Jones

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  1. Roger A Jones
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© 1994 Humana Press Inc., Totowa, NJ

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Jones, R.A. (1994). Synthesis of [15N]-Labeled DNA Fragments. In: Protocols for Oligonucleotide Conjugates. Methods in Molecular Biology, vol 26. Humana Press. https://doi.org/10.1007/978-1-59259-513-6_8

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  • DOI: https://doi.org/10.1007/978-1-59259-513-6_8

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-252-1

  • Online ISBN: 978-1-59259-513-6

  • eBook Packages: Springer Protocols

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