The realization that the oligosaccharide moieties of glycoconjugates, such as glycoproteins and glycolipids, are involved in important intra- and intercellular of important oligosaccharide structures as tools in analytical and therapeutic studies (see refs. 1 and 2 for reviews). A number of efficient chemical procedures have been developed for the synthesis of the “glyco-” part (see refs. 3–7 for review). However, for the synthesis of a given saccharide structure, an individual strategy has to be set up comprising many laborious protection and deprotection steps for stereo-controlled synthesis, which finally results in only moderate overall yields. As an example, the synthesis of N-acetyllactosamine (LacNAc) involves 12 steps and needs 3 months of lab work (7). A scale-up of the chemical procedure often encounters environmental problems because all steps are carried out in organic solvents.
- Enzymatic Synthesis
- Nucleotide Sugar
- Product Solution
- Potassium Acetate
- Product Isolation
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© 1999 Humana Press Inc., Totowa, NJ
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Cite this protocol
Zervosen, A., Elling, L. (1999). Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides. In: Bucke, C. (eds) Carbohydrate Biotechnology Protocols. Methods in Biotechnology™, vol 10. Humana Press. https://doi.org/10.1007/978-1-59259-261-6_19
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Print ISBN: 978-0-89603-563-8
Online ISBN: 978-1-59259-261-6
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