Skip to main content
SpringerLink
Log in

Competitive ELISA

  • Protocol
Immunochemical Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 80))

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a very useful technique for the specific and sensitive assay of certain compounds, in which suitable antibodies, monoclonal or polyclonal, to the compounds are available. The technique has found particular application in the monitoring of environmental contaminants and toxins, either studying the primarily contaminated materials, e.g., foodstuffs, or body fluids of potentially exposed humans. The technique has been increasingly applied to monitoring the carcinogenic mycotoxins, the aflatoxins.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Sizaret, P., Malaveilie, C., Montesano, R., and Frayssinet, C. (1982) Detection of aflatoxins and related metabolites by radioimmunoassay. J. Natl. Cancer Inst. 69, 1375–1381.

    PubMed  CAS  Google Scholar 

  2. Lowry, O. H., Rosebrough, N. J., Fan, A. L., and Randall, L. J. (1961) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.

    Google Scholar 

  3. Waterborg, J H and Matthews, H. R. (1984) The Lowry method for protein quantitation, in Methods in Molecular Biology, vol 1 (Walker, J., ed), Humana, Clifton, NJ, pp. 1–3

    Google Scholar 

  4. Martin, C. N., Gamer, R. C., Tursi, F., Gamer, J. V., Whittle, H. C, Ryder, R. W., Sizaret, P., and Montesano, R. (1984) An enzyme linked immunosorbent procedure for assaying aflatoxin B1, in Monitoring Human Exposure to Carcinogenic and Mutagenic Agents (Berlin, A., Draper, M, Hemminki, K., and Vaimo, H., eds.), IARC Scientific Publication No. 59, IARC, Lyon, France, pp. 313–321.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology Faculty of Science, Mahidol University, Bangkok, Thailand

    Kittima Makarananda

  2. MRC Toxicology Unit, University of Leicester, UK

    Lucinda R. Weir & Gordon E. Neal

Authors
  1. Kittima Makarananda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lucinda R. Weir
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gordon E. Neal
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The University of Birmingham, UK

    John D. Pound

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Makarananda, K., Weir, L.R., Neal, G.E. (1998). Competitive ELISA. In: Pound, J.D. (eds) Immunochemical Protocols. Methods in Molecular Biology™, vol 80. Humana Press. https://doi.org/10.1007/978-1-59259-257-9_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-59259-257-9_15

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-493-8

  • Online ISBN: 978-1-59259-257-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation