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Microwave Fixation of Rat Hippocampal Slices

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Microwave Techniques and Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The authors’ research focuses on the structural basis of synaptic function. Knowing the dimensions and connectivity of presynaptic and postsynaptic elements is necessary for understanding the mechanisms of synaptic transmission in the central nervous system. To this end, electrophysiological (EP) methods are used in combination with confocal microscopy, serial electron microscopy (EM), three-dimensional reconstruction, and quantitative image analysis. Microwave (MW)-enhanced methods for preparing hippocampal (HC) slices are described in this chapter. These procedures have been adapted from (1989) and (1985).

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Author information

Authors and Affiliations

  1. Department of Biology, Boston University, Boston, MA

    Marcia D. Feinberg, Karen M. Szumowski & Kristen M. Harris

Authors
  1. Marcia D. Feinberg
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  2. Karen M. Szumowski
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  3. Kristen M. Harris
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Editor information

Editors and Affiliations

  1. Ted Pella Inc., Redding, CA

    Richard T. Giberson MS

  2. California State University, Chico, CA

    Richard S. Demaree Jr. PhD

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© 2001 Humana Press Inc., Totowa, NJ

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Feinberg, M.D., Szumowski, K.M., Harris, K.M. (2001). Microwave Fixation of Rat Hippocampal Slices. In: Giberson, R.T., Demaree, R.S. (eds) Microwave Techniques and Protocols. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-128-2_7

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  • DOI: https://doi.org/10.1007/978-1-59259-128-2_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-903-2

  • Online ISBN: 978-1-59259-128-2

  • eBook Packages: Springer Book Archive

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