Abstract
Ultrastructural studies usually require that the sample be sacrificed and the tissue of interest be prepared by specialized fixation procedures. The goal of any given ultrastructural study is to observe and record the distribution and organization of a cellular constituent(s), in a way that most closely represents its in vivo distribution. Chemical fixations with aldehydes or cold solvents are often employed to preserve specimens for ultrastructural studies. However, these studies are only “snapshots” in time of the chemical termination of the sample. There can often be difficulties in interpreting the results, because of the harsh manipulations that chemical fixations can induce upon the specimen. Another practical disadvantage of chemical fixation in the preparation of biological specimens for microscopy is the time required to complete the process.
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References
Boon ME, Hendrikse FCJ, Kok PG, Bolhuis P, Kok LP (1990) Practical approach to routine immunostaining of paraffin sections in the microwave oven. Histochem J 22:347–352.
Cooper MS, D’Amico LA, Henry CA (1999) Confocal microscopic analysis of morphogenetic movements. Methods Cell Biol 59:179–204.
Frey T (1995) Nucleic acid dyes for detection of apoptosis in live cells. Cytometry 21:265–274.
Giberson RT, Demaree RS Jr. (1995) Microwave fixation: understanding the variables to achieve rapid reproducible results. Microsc Res Tech 32:246–254.
Gu J (1994) Microwave Immunocytochemistry. In Gu J, Hacker GW, eds, Modern Methods in Analytical Morphology. Plenum, New York, pp.67–80.
Gu J, Choi T-S, Whittlesey M, Slap S, Anderson V (1995) Development of microwave immunohistochemistry. Cell Vision 2:257–259.
Login GR, Dvorak AM (1993) Review of rapid microwave fixation technology: its expanding niche in morphological studies. Scanning 15:58–66.
Mayers CP (1970) Histological fixation by microwave heating. J Clin Pathol 23:273–275.
Minden JS, Agard DA, Sedat JW, Alberts BM (1989) Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos. J Cell Biol 109:505–516.
Stone JR, Walker WA, Povlishock JT (1999) Visualization of a new class of tramatically injured axons through the use of a modified method of microwave antigen retrieval. Acta Neuropathol 97:335–345.
Sullivan W, Minden JS, Alberts BM (1990) Daughterless-abo-like, a Drosophila mater-nal-effect mutation that exhibits abnormal centrosome separation during the late blastoderm divisions. Development 110:311–323.
Tornehave D, Hougaard DM, Larsson L-I (2000) Microwaving for double indirect immunofluorescence with primary antibodies from the same species and for staining of mouse tissues with mouse monoclonal antibodies. Histochem Cell Biol 113:19–23.
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© 2001 Humana Press Inc., Totowa, NJ
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Sanders, M.A., Gartner, D.M. (2001). In Vivo Microwave-Assisted Labeling of Allium and Drosophila Nuclei. In: Giberson, R.T., Demaree, R.S. (eds) Microwave Techniques and Protocols. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-128-2_12
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DOI: https://doi.org/10.1007/978-1-59259-128-2_12
Publisher Name: Humana Press
Print ISBN: 978-0-89603-903-2
Online ISBN: 978-1-59259-128-2
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