Abstract
High-performance tangential flow filtration (HPTFF) is an emerging technology that uses semipermeable membranes for the separation of proteins without limit to their relative size (1). HPTFF can be used throughout the purification process to remove specific impurities (e.g., proteins, DNA, or endotoxins), clear viruses, and/or eliminate protein oligomers or degradation products. In addition, HPTFF can effect simultaneous purification, concentration, and buffer exchange, providing the unique capability of combining several different separation steps into a single scalable unit operation.
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Zydney, A.L., Kuriyel, R. (2000). 4 High-Performance Tangential Flow Filtration for Protein Separations. In: Desai, M.A. (eds) Downstream Processing of Proteins. Methods in Biotechnology, vol 9. Humana Press. https://doi.org/10.1007/978-1-59259-027-8_4
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DOI: https://doi.org/10.1007/978-1-59259-027-8_4
Publisher Name: Humana Press
Print ISBN: 978-0-89603-564-5
Online ISBN: 978-1-59259-027-8
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