Abstract
The disruption of a cell’s wall is often a primary step in product isolation, particularly when hosts such as Escherichia coli and Saccharomyces cerevisiae, which generally do not excrete product, are employed. Of the available methods, high-pressure homogenization is dominant at moderate or large process volumes.
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Middelberg, A.P.J. (2000). 2 Microbial Cell Disruption by High-Pressure Homogenization. In: Desai, M.A. (eds) Downstream Processing of Proteins. Methods in Biotechnology, vol 9. Humana Press. https://doi.org/10.1007/978-1-59259-027-8_2
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DOI: https://doi.org/10.1007/978-1-59259-027-8_2
Publisher Name: Humana Press
Print ISBN: 978-0-89603-564-5
Online ISBN: 978-1-59259-027-8
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