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Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters

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Plant and Food Carotenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2083))

Abstract

Apocarotenoids are carotenoid derivatives produced by the nonenzymatic or enzymatic cleavage of carotenoids, followed by different enzymatic modifications. In plants, apocarotenoids play different roles, such as attraction of pollinators and seeds dispersal, defense against pathogens and herbivores, protection against photo-oxidative stresses, stimulation and inhibition of plant growth and regulation of biological processes in the case of phytohormones abscisic acid and strigolactones. While carotenoids are in general plastid-localized metabolites, apocarotenoids can reach different final destinations inside or outside the cell. The mechanisms of apocarotenoid transport through biological membranes have been poorly studied. This chapter describes a method to characterize transmembrane transporters involved in the transport of polar and amphipathic apocarotenoids. This protocol was successfully used to in vitro characterize the transport activity of ATP-binding cassette (ABC) and multidrug and toxic extrusion (MATE) in microsomes isolated from Saccharomyces cerevisiae expressing these plant transporters.

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Acknowledgments

Work in GG’s lab was partially supported by the European Union’s Horizon 2020 programme, project “Newcotiana”, Grant Agreement 760331, and by a grant from the Lazio Region, project “ProBioZaff”.

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Author notes

  1. Olivia Costantina Demurtas and Rita de Brito Francisco contributed equally to this work.

Authors and Affiliations

  1. ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, C.R. Casaccia, Rome, Italy

    Olivia Costantina Demurtas & Giovanni Giuliano

  2. Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland

    Rita de Brito Francisco & Enrico Martinoia

Authors
  1. Olivia Costantina Demurtas
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  2. Rita de Brito Francisco
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  3. Enrico Martinoia
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  4. Giovanni Giuliano
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Corresponding author

Correspondence to Giovanni Giuliano .

Editor information

Editors and Affiliations

  1. CSIC-IRTA-UAB-UB, Centre for Research in Agricultural Genomics (CRAG), Barcelona, Spain

    Manuel Rodríguez-Concepción

  2. Institute for Biology II, University of Freiburg, Freiburg, Germany

    Ralf Welsch

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Demurtas, O.C., de Brito Francisco, R., Martinoia, E., Giuliano, G. (2020). Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters. In: Rodríguez-Concepción, M., Welsch, R. (eds) Plant and Food Carotenoids. Methods in Molecular Biology, vol 2083. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9952-1_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9952-1_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9951-4

  • Online ISBN: 978-1-4939-9952-1

  • eBook Packages: Springer Protocols

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