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A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants

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Plant and Food Carotenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2083))

Abstract

We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.

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Acknowledgments

This work was supported by base line funding and a Competitive Research Grant (CRG4) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).

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Authors and Affiliations

  1. The BioActives Lab, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia

    Jianing Mi, Kun-Peng Jia, Aparna Balakrishna & Salim Al-Babili

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  1. Jianing Mi
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  2. Kun-Peng Jia
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  3. Aparna Balakrishna
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  4. Salim Al-Babili
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Corresponding author

Correspondence to Salim Al-Babili .

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Editors and Affiliations

  1. CSIC-IRTA-UAB-UB, Centre for Research in Agricultural Genomics (CRAG), Barcelona, Spain

    Manuel Rodríguez-Concepción

  2. Institute for Biology II, University of Freiburg, Freiburg, Germany

    Ralf Welsch

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Mi, J., Jia, KP., Balakrishna, A., Al-Babili, S. (2020). A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants. In: Rodríguez-Concepción, M., Welsch, R. (eds) Plant and Food Carotenoids. Methods in Molecular Biology, vol 2083. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9952-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9952-1_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9951-4

  • Online ISBN: 978-1-4939-9952-1

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