Abstract
Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.
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Lang, T. (2020). Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells. In: Harris, J., Morand, E. (eds) Macrophage Migration Inhibitory Factor. Methods in Molecular Biology, vol 2080. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9936-1_4
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DOI: https://doi.org/10.1007/978-1-4939-9936-1_4
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9935-4
Online ISBN: 978-1-4939-9936-1
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