Abstract
Transposable elements can be highly mutagenic because when they transpose they can insert into genes and disrupt their function, a propensity which has been exploited in many organisms to generate tagged mutant alleles. The Mutator (Mu) family transposon is a family of DNA-type transposons in maize with a particularly high duplication frequency, which results in large numbers of new mutations in lineages that carry active Mu elements. Here we describe a rapid and cost-effective Miseq-based Mu transposon profiling pipeline. This method can also be used for identifying flanking sequences of other types of long insertions such as T-DNAs.
Key words
- Transposon
- Mutator
- Insertion profiling
- Next-generation sequencing (NGS)
- Target site duplication
- Terminal inverted repeats
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Acknowledgments
This work was supported by the National Science Foundation Grant DBI-1237931 and Purdue Startup Funds to D.L.
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Zhang, X., Zhao, M., Lisch, D. (2020). Cost-Effective Profiling of Mutator Transposon Insertions in Maize by Next-Generation Sequencing. In: Vaschetto, L. (eds) Cereal Genomics. Methods in Molecular Biology, vol 2072. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9865-4_5
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DOI: https://doi.org/10.1007/978-1-4939-9865-4_5
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