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Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of Their Sporozoites for Downstream Manipulation

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Toxoplasma gondii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2071))

Abstract

Toxoplasma gondii tachyzoites and bradyzoites are studied extensively in the laboratory due to the ease with which they can be cultured. In contrast, oocysts and the sporozoites within them are more difficult to work with, in that cat infections are required for their generation and isolating sporozoites requires a laborious excystation procedure. More over some parasite species such as Hammondia hammondi are obligately heteroxenous and require passage through a cat for completion of the life cycle. There is no debate that there is great value in studying this important life cycle stage, and we present here a detailed description of the current protocols used in our laboratories to generate and isolate T. gondii and H. hammondi oocysts, and to excyst and purify the sporozoites within them for use in downstream experimental applications.

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Author notes

  1. Sarah L. Sokol and Zhee Sheen Wong contributed equally to this work.

Authors and Affiliations

  1. Department of Biological Sciences, Dietrich School of Arts and Sciences, University of Pittsburgh, Pittsburgh, PA, USA

    Sarah L. Sokol, Zhee Sheen Wong & Jon P. Boyle

  2. Agricultural Research Service, Animal Parasitic Diseases Laboratory, United States Department of Agriculture (USDA), Beltsville Agricultural Research Center, Beltsville, MD, USA

    Jitender P. Dubey

Authors
  1. Sarah L. Sokol
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  2. Zhee Sheen Wong
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  3. Jon P. Boyle
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  4. Jitender P. Dubey
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Corresponding authors

Correspondence to Jon P. Boyle or Jitender P. Dubey .

Editor information

Editors and Affiliations

  1. Division of Infectious Disease and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia

    Christopher J. Tonkin

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Sokol, S.L., Wong, Z.S., Boyle, J.P., Dubey, J.P. (2020). Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of Their Sporozoites for Downstream Manipulation. In: Tonkin, C. (eds) Toxoplasma gondii. Methods in Molecular Biology, vol 2071. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9857-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9857-9_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9856-2

  • Online ISBN: 978-1-4939-9857-9

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