Abstract
Methicillin-susceptible S. aureus changes to methicillin-resistant S. aureus (MRSA) upon the acquisition of staphylococcal cassette chromosome mec (SCCmec), a genomic island that encodes methicillin resistance. SCCmec elements in S. aureus are classified into different types based on the combination of mec gene complexes and ccr gene complexes, which share variations, five classes in mec and eight in ccr. To date, at least 13 types of SCCmec elements have been identified and each SCCmec type has individual characteristics. It is known that hospital-associated MRSA strains carry SCCmec elements of types, I, II, and III, and the majority of community-acquired MRSA strains carry characteristic SCCmec elements, type IV SCCmec or type V SCCmec. We herein describe multiplex PCR methods to type SCCmec elements by identifying the mec gene complex class and ccr gene complex type.
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References
Ito T, Kuwahara-Arai K, Katayama Y, Uehara Y, Han X, Kondo Y, Hiramatsu K (2014) Staphylococcal cassette chromosome mec (SCCmec) analysis of MRSA. Methods Mol Biol 1085:131–148
Ito T, Katayama Y, Hiramatsu K (1999) Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob Agents Chemother 43(6):1449–1458
Katayama Y, Ito T, Hiramatsu K (2000) A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 44(6):1549–1555
International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements IWG (2009) Classification of staphylococcal cassette chromosome mec (SCCmec): guidelines for reporting novel SCCmec elements. Antimicrob Agents Chemother 53(12):4961–4967
Ito T, Katayama Y, Asada K, Mori N, Tsutsumimoto K, Tiensasitorn C et al (2001) Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 45(5):1323–1336
Ma XX, Ito T, Tiensasitorn C, Jamklang M, Chongtrakool P, Boyle-Vavra S (2002) Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob Agents Chemother 46(4):1147–1152
Ito T, Ma XX, Takeuchi F, Okuma K, Yuzawa H, Hiramatsu K (2004) Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob Agents Chemother 48(7):2637–2651
Oliveira DC, Milheirico C, de Lencastre H (2006) Redefining a structural variant of staphylococcal cassette chromosome mec, SCCmec type VI. Antimicrob Agents Chemother 50(10):3457–3459
Berglund C, Ito T, Ikeda M, Ma XX, Soderquist B, Hiramatsu K (2008) Novel type of staphylococcal cassette chromosome mec in a methicillin-resistant Staphylococcus aureus strain isolated in Sweden. Antimicrob Agents Chemother 52(10):3512–3516
Zhang K, McClure JA, Elsayed S, Conly JM (2009) Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 53(2):531–540
Li S, Skov RL, Han X, Larsen AR, Larsen J, Sorum M et al (2011) Novel types of staphylococcal cassette chromosome mec elements identified in clonal complex 398 methicillin-resistant Staphylococcus aureus strains. Antimicrob Agents Chemother 55(6):3046–3050
Garcia-Alvarez L, Holden MT, Lindsay H, Webb CR, Brown DF, Curran MD et al (2011) Methicillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infect Dis 11(8):595–603
Wu Z, Li F, Liu D, Xue H, Zhao X (2015) Novel type XII staphylococcal cassette chromosome mec harboring a new cassette chromosome recombinase, CcrC2. Antimicrob Agents Chemother 59(12):7597–7601
Baig S, Johannesen TB, Overballe-Petersen S, Larsen J, Larsen AR, Stegger M (2018) Novel SCCmec type XIII (9A) identified in an ST152 methicillin-resistant Staphylococcus aureus. Infect Genet Evol 61:74–76
Katayama Y, Ito T, Hiramatsu K (2001) Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus. Antimicrob Agents Chemother 45(7):1955–1963
Ryffel C, Bucher R, Kayser FH, Berger-Bachi B (1991) The Staphylococcus aureus mec determinant comprises an unusual cluster of direct repeats and codes for a gene product similar to the Escherichia coli sn-glycerophosphoryl diester phosphodiesterase. J Bacteriol 173(23):7416–7422
Goering RV, Morrison D, Al-Doori Z, Edwards GF, Gemmell CG (2008) Usefulness of mec-associated direct repeat unit (dru) typing in the epidemiological analysis of highly clonal methicillin-resistant Staphylococcus aureus in Scotland. Clin Microbiol Infect 14(10):964–969
Ito T, Hiramatsu K, Tomasz A, de Lencastre H, Perreten V, Holden MT et al (2012) Guidelines for reporting novel mecA gene homologues. Antimicrob Agents Chemother 56(10):4997–4999
Chambers HF (2001) The changing epidemiology of Staphylococcus aureus? Emerg Infect Dis 7(2):178–182
Eady EA, Cove JH (2003) Staphylococcal resistance revisited: community-acquired methicillin resistant Staphylococcus aureus--an emerging problem for the management of skin and soft tissue infections. Curr Opin Infect Ds 16(2):103–124
Okuma K, Iwakawa K, Turnidge JD, Grubb WB, Bell JM, O’Brien FG et al (2002) Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. J Clin Microbiol 40(11):4289–4294
Chambers HF, Deleo FR (2009) Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol 7(9):629–641
Schijffelen MJ, Boel CH, van Strijp JA, Fluit AC (2010) Whole genome analysis of a livestock-associated methicillin-resistant Staphylococcus aureus ST398 isolate from a case of human endocarditis. BMC Genomics 11:376
Shore AC, Deasy EC, Slickers P, Brennan G, O’Connell B, al MS (2011) Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 55(8):3765–3773
Han X, Ito T, Takeuchi F, Ma XX, Takasu M, Uehara Y et al (2009) Identification of a novel variant of staphylococcal cassette chromosome mec, type II.5, and its truncated form by insertion of putative conjugative transposon Tn6012. Antimicrob Agents Chemother 53(6):2616–2619
Kondo Y, Ito T, Ma XX, Watanabe S, Kreiswirth BN, Etienne J et al (2007) Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother 51(1):264–274
Chongtrakool P, Ito T, Ma XX, Kondo Y, Trakulsomboon S, Tiensasitorn C et al (2006) Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrob Agents Chemother 50(3):1001–1012
Berglund C, Ito T, Ma XX, Ikeda M, Watanabe S, Soderquist B et al (2009) Genetic diversity of methicillin-resistant Staphylococcus aureus carrying type IV SCCmec in Orebro County and the western region of Sweden. J Antimicrob Chemother 63(1):32–41
Kwon NH, Park KT, Moon JS, Jung WK, Kim SH, Kim JM et al (2005) Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J Antimicrob Chemother 56(4):624–632
Milheirico C, Oliveira DC, de Lencastre H (2007) Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus: ‘SCCmec IV multiplex’. J Antimicrob Chemother 60(1):42–48
Cavaco LM, Hasman H, Stegger M, Andersen PS, Skov R, Fluit AC et al (2010) Cloning and occurrence of czrC, a gene conferring cadmium and zinc resistance in methicillin-resistant Staphylococcus aureus CC398 isolates. Antimicrob Agents Chemother 54(9):3605–3608
Oliveira DC, de Lencastre H (2002) Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 46(7):2155–2161
Zhang K, McClure JA, Elsayed S, Louie T, Conly JM (2005) Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 43(10):5026–5033
Zhang K, McClure JA, Conly JM (2012) Enhanced multiplex PCR assay for typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. Mol Cell Probes 26(5):218–221
Milheirico C, Oliveira DC, de Lencastre H (2007) Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus. Antimicrob Agents Chemother 51(9):3374–3377
Oliveira DC, Wu SW, de Lencastre H (2000) Genetic organization of the downstream region of the mecA element in methicillin-resistant Staphylococcus aureus isolates carrying different polymorphisms of this region. Antimicrob Agents Chemother 44(7):1906–1910
Kaya H, Hasman H, Larsen J, Stegger M, Johannesen TB, Allesoe RL et al (2018) SCCmecFinder, a web-based tool for typing of staphylococcal cassette chromosome mec in Staphylococcus aureus using whole-genome sequence data. mSphere 3(1). https://doi.org/10.1128/mSphere.00612-17
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual - 2nd ed. Cold Spring Harbor Laboratory Press, New York, NY
Lee AS, de Lencastre H, Garau J, Kluytmans J, Malhotra-Kumar S, Peschel A et al (2018) Methicillin-resistant Staphylococcus aureus. Nat Rev Dis Primers 4:18033
Acknowledgments
We sincerely thank Yuki Katayama, Keiichi Hiramatsu, Herminia de Lencastre, Bo Soderquist, Kunyan Zhang, Anders Rhod Larsen, and Mark Holmes for providing some reference strains used in this study. Furthermore, I would like to take this opportunity to express my sincere appreciation and gratitude to late Teruyo Ito for her great assistance.
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Yamaguchi, T., Ono, D., Sato, A. (2020). Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 2069. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9849-4_4
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DOI: https://doi.org/10.1007/978-1-4939-9849-4_4
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