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Diabetic Nephropathy pp 205–240Cite as

Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2067))

Abstract

Multiple genetic strategies are available to help improve understanding of diabetic nephropathy. This methods chapter provides an overview of phenotype considerations specific to diabetic nephropathy and biobank essentials, and provides detailed methodology for a common benchtop wet-lab approach (Ion Torrent semiconductor sequencing) including in silico genetic variant identification from next-generation sequencing data to identify genetic risk factors for diabetic nephropathy.

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Authors and Affiliations

  1. Centre for Public Health, Queen’s University Belfast, Northern Ireland, UK

    Laura J. Smyth, Katie Kerr, Seamus Duffy, Jill Kilner & Amy Jayne McKnight

Authors
  1. Laura J. Smyth
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  2. Katie Kerr
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  3. Seamus Duffy
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  4. Jill Kilner
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  5. Amy Jayne McKnight
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Corresponding author

Correspondence to Amy Jayne McKnight .

Editor information

Editors and Affiliations

  1. Faculty of Life Sciences & Medicine School of Cardiovascular Medicine & Sciences, British Heart Foundation Centre of Research Excellence, King’s College London, London, UK

    Luigi Gnudi

  2. Developmental Biology and Cancer Programme, UCL Great Ormond Street Institute of Child Health, University College London, London, UK

    David A. Long

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Smyth, L.J., Kerr, K., Duffy, S., Kilner, J., McKnight, A.J. (2020). Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches. In: Gnudi, L., Long, D. (eds) Diabetic Nephropathy. Methods in Molecular Biology, vol 2067. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9841-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9841-8_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9840-1

  • Online ISBN: 978-1-4939-9841-8

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