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Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus

  • Julia M. Draper
  • Jay L. VivianEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2066)

Abstract

Induced pluripotent stem (iPS) cells are important tools for studying differentiation and for use in patient-specific disease modeling. We present a detailed method for the reprogramming of primary human fibroblasts to induced pluripotent stem cells using Sendai virus. These procedures allow for the efficient generation of multiple high-quality feeder-independent iPS cell lines for a given human fibroblast line. The iPS cell lines generated by this protocol can be used in a variety of differentiation and gene expression studies, as well as in genetic manipulations.

Key words

Human pluripotent stem cells Induced pluripotent stem cells Reprogramming Primary cell Sendai virus Fibroblast 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Transgenic and Gene-Targeting Institutional FacilityUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.Department of Pathology and Laboratory MedicineUniversity of Kansas Medical CenterKansas CityUSA

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