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Breeding Strategies for Genetically Modified Mice

  • Jan Parker-ThornburgEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2066)

Abstract

Genetically modified mice can be generated using a variety of methods. Depending on the method used, the breeding strategy must be modified not only for the initial founding generation of mouse, but to establish individual mouse lines as well. Transgenic founder mice are each unique and must be outcrossed to initially establish the line. Mice that have been targeted using embryonic stem cells will need to be tested for germ line transmission of the modified gene prior to establishing a line. Mice that have been targeted using CRISPR/Cas9 gene-editing endonucleases will often be mosaic, and thus, also require testing for germ line transmission of the mutation of interest.

Key words

Transgenic Knock-out mice Knock-in mice CRISPR CRISPR/Cas9 Germ line transmission Chimera Mosaic 

Notes

Acknowledgments

This work was supported by NCI grant #CA016672 (GEMF), a Cancer Center Support Grant to MD Anderson Cancer Center and NCI grant #R50CA211121.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Genetically Engineered Mouse Facility, Department of GeneticsMD Anderson Cancer CenterHoustonUSA

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