Sperm-Mediated Genetic Modifications

  • Marialuisa LavitranoEmail author
  • Laura Farina
  • Maria Grazia Cerrito
  • Roberto Giovannoni
Part of the Methods in Molecular Biology book series (MIMB, volume 2066)


The ability to introduce controlled modifications of the genome of animals represents an important tool for biomedical and veterinary research. Among transgenic techniques, we describe here the sperm-mediated gene transfer method that is based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to the oocyte during fertilization, producing genetically modified animals.

Key words

Spermatozoa Transgenic animals SMGT Transgenesis Mouse 



This work was supported by the Italian Minister of Research and University (FIRB, RBAP06LAHL).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Marialuisa Lavitrano
    • 1
    Email author
  • Laura Farina
    • 1
  • Maria Grazia Cerrito
    • 1
  • Roberto Giovannoni
    • 1
  1. 1.School of Medicine and SurgeryUniversity of Milano-BicoccaMonzaItaly

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