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QuantStudio 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis

  • Chiara Broccanello
  • Letizia Gerace
  • Piergiorgio StevanatoEmail author
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Part of the Methods in Molecular Biology book series (MIMB, volume 2065)

Abstract

Real time technology provides great advancements over PCR-based methods for a broad range of applications. With the increased availability of sequencing information, there is a need for the development and application of high-throughput real time PCR genotyping and gene expression methods that significantly broaden the current screening capabilities. Thermo Fisher Scientific (USA) has released a platform (QuantStudio™ 12K Flex system coupled with OpenArray® technology) with key elements required for high-throughput SNP genotyping and gene expression analysis. This allows for a rapid screening of large numbers of TaqMan® assays (up to 256) in many samples (up to 480) per run. This advanced real-time method involves the use of an array composed of 3,000 through-holes running on the QuantStudio™ 12K with OpenArray® block. The aim of this chapter is to outline the OpenArray® approach while providing a comprehensive in-depth review of the scientific literature on this topic. In agreement with a large number of independent studies, we conclude that the use of OpenArray® technology is a rapid and accurate method for high-throughput and large-scale systems biology studies with high specificity and sensitivity.

Key words

TaqMan® assay OpenArray® plate SNP genotyping Association analysis Gene and miRNA expression profiling 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Chiara Broccanello
    • 1
  • Letizia Gerace
    • 2
  • Piergiorgio Stevanato
    • 1
    Email author
  1. 1.DAFNAEUniversità Degli Studi di PadovaLegnaro (PD)Italy
  2. 2.Thermo Fisher Scientific, Life Sciences SolutionsMonza (MB)Italy

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