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Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR

  • Francesca Salvianti
  • Filomena Costanza
  • Gemma Sonnati
  • Pamela PinzaniEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2065)

Abstract

We propose two different approaches involving the use of quantitative real-time PCR for the detection or analysis of circulating tumor cells. In one case cells are indirectly identified through the expression of a marker mRNA, while in the other one cells are enriched by size prior to be submitted to mutational analysis for a specific target. Both methods have been successfully applied to the study of circulating melanoma cells.

Key words

CTCs Melanoma RT-qPCR Tyrosinase qPCR Somatic mutation BRAFV600E 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Francesca Salvianti
    • 1
  • Filomena Costanza
    • 1
  • Gemma Sonnati
    • 1
  • Pamela Pinzani
    • 1
    Email author
  1. 1.Department of Experimental and Clinical Biomedical Sciences “Mario Serio”University of FlorenceFlorenceItaly

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