A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis

  • Maria Ada Prusicki
  • Yuki Hamamura
  • Arp SchnittgerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2061)


Plants are powerful model systems to study meiosis. Our knowledge about the cytology of plant meiosis is mainly based on the analysis of fixed material. Although highly informative, this approach is limited in understanding the dynamics of meiosis. Here, we present a step-by-step instruction for a newly developed method to follow meiosis in male meiocytes of Arabidopsis in real time by confocal laser scanning microscopy. We envision that this method can be easily translated to other plant species and especially crops (e.g., Brassica, maize, and potato).

Key words

Meiosis Arabidopsis Confocal laser scanning microscopy Live cell imaging Time lapse 



This work was supported by the EU-ITN grant COMREC, the BMBF grant TarBreed, and core funding of the University of Hamburg to A.S.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Maria Ada Prusicki
    • 1
  • Yuki Hamamura
    • 1
  • Arp Schnittger
    • 1
    Email author
  1. 1.Department of Developmental BiologyUniversity of HamburgHamburgGermany

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