Abstract
The translational success of oncolytic virotherapies would benefit from the widespread use of clinically relevant ex vivo models. Malignant ascites, an accumulation of fluid in the peritoneum due to disseminated cancer, recapitulates many features of the tumor microenvironment, making it a valuable model for studying oncolytic virus activity. Here, we describe a method for the separation and storage of cellular and acellular components of malignant ascites, followed by flow cytometric characterization of the cellular fraction. We then outline a simple experiment using whole ascites to assess the activity of a bispecific T cell engager (BiTE)-expressing oncolytic adenovirus.
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Acknowledgments
This work was supported by grants from Cancer Research UK (C552/A17720 and C5255/A20936) and the Medical Research Council (MR/N013468/1). JLR is supported in part by the Sir Paul Nurse Junior Research Fellowship at Linacre College. The studies described in this protocol were performed under an ethics permission from the Oxford Radcliffe Biobank (16/A070).
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Scott, E.M., Frost, S., Khalique, H., Freedman, J.D., Seymour, L.W., Lei-Rossmann, J. (2020). Use of Liquid Patient Ascites Fluids as a Preclinical Model for Oncolytic Virus Activity. In: Engeland, C. (eds) Oncolytic Viruses. Methods in Molecular Biology, vol 2058. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9794-7_17
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DOI: https://doi.org/10.1007/978-1-4939-9794-7_17
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9794-7
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