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FISH Protocol for Myotonic Dystrophy Type 1 Cells

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Trinucleotide Repeats

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2056))

Abstract

Mutant DMPK transcripts containing expanded CUG repeats (CUGexp) are retained within the nucleus of myotonic dystrophy type 1 (DM1) cells as discrete foci. Nuclear CUGexp-RNA foci that sequester MBNL1 splicing factor represent a hallmark of this RNA dominant disease caused by the expression of expanded microsatellite repeats. Here we described fluorescent in situ hybridization (FISH) techniques to detect either RNA containing CUG expansion or DMPK transcripts in human DM1 or WT cells. In addition, we propose a combined FISH/immunofluorescence protocol to visualize the colocalization of MBNL1 with CUGexp-RNA foci in DM1 cells.

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Acknowledgments

This research was supported by Sorbonne Université, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS), and the Association Institut de Myologie (AIM).

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Authors and Affiliations

  1. Centre de Recherche en Myologie, Sorbonne Univeristé, Paris, France

    Arnaud F. Klein, Ludovic Arandel, Joelle Marie & Denis Furling

  2. Centre de Recherche en Myologie, Inserm, Paris, France

    Arnaud F. Klein, Ludovic Arandel, Joelle Marie & Denis Furling

  3. Association Institut de Myologie, Paris, France

    Arnaud F. Klein, Ludovic Arandel, Joelle Marie & Denis Furling

Authors
  1. Arnaud F. Klein
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  2. Ludovic Arandel
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  3. Joelle Marie
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  4. Denis Furling
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Corresponding author

Correspondence to Denis Furling .

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Editors and Affiliations

  1. Department Genomes & Genetics, Institut Pasteur, Paris, France

    Guy-Franck Richard

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Klein, A.F., Arandel, L., Marie, J., Furling, D. (2020). FISH Protocol for Myotonic Dystrophy Type 1 Cells. In: Richard, GF. (eds) Trinucleotide Repeats. Methods in Molecular Biology, vol 2056. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9784-8_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9784-8_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9783-1

  • Online ISBN: 978-1-4939-9784-8

  • eBook Packages: Springer Protocols

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