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Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture

  • Gregory M. Williams
  • Robert S. LahueEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2056)

Abstract

Determining the molecular mechanisms that contribute to trinucleotide repeat (TNR) expansions is essential to understanding the origin of genetically inherited diseases, such as Huntington’s disease, and to inform efforts in developing therapeutic treatments. As one resource to probe the mechanisms of TNR expansions, we describe an expansion assay in human tissue culture cells. The cell line SVG-A, derived from human astrocytes, has the important property of supporting expansions in culture, unlike many cell lines derived from patients. SVG-A cells are also amenable to standard genetic and biochemical techniques such as siRNA, CRISPR-Cas9 and enzymatic inhibitors. This combination of features allows for mechanistic studies of TNR expansions, using the quantitative genetic assay described here as a readout. The SVG-A assay has correctly identified key proteins that drive expansions and it has facilitated testing of enzymatic inhibitors that suppress expansions as potential therapeutics. This chapter describes how repeat expansions are detected, visualized, and quantified.

Keywords

Trinucleotide repeats Expansion Genome instability DNA mutagenesis Huntington’s disease Reporter construct Polymerase chain reaction Astrocytes Human cells 

Notes

Acknowledgments

This work was supported by Government of Ireland Postdoctoral Fellowship GOIPD/2018/291 from the Irish Research Council (to Gregory M. Williams) and by Science Foundation Ireland grant 16/BBSRC/3395 (to Robert S. Lahue).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Centre for Chromosome BiologyNational University of Ireland, GalwayGalwayIreland
  2. 2.Galway Neuroscience CentreNational University of Ireland, GalwayGalwayIreland

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