Theranostics pp 171-183 | Cite as

Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies

  • Laura V. CroftEmail author
  • Jaimie A. Mulders
  • Derek J. Richard
  • Kenneth O’Byrne
Part of the Methods in Molecular Biology book series (MIMB, volume 2054)


Digital holographic imaging (DHI) is a noninvasive, live cell imaging technique that enables long-term quantitative visualization of cells in culture. DHI uses phase-shift imaging to monitor and quantify cellular events such as cell division, cell death, cell migration, and drug responses. In recent years, the application of DHI has expanded from its use in the laboratory to the clinical setting, and currently it is being developed for use in theranostics. Here, we describe the use of the DHI platform HoloMonitorM4 to evaluate the effects of novel, targeted cancer therapies on cell viability and proliferation using the HeLa cancer cell line as a model. We present single cell tracking and population-wide analysis of multiple cell morphology parameters.

Key words

Digital holographic imaging Noninvasive live cell imaging Label-free live cell imaging Apoptosis imaging Drug effect analysis HolomonitorM4 Cytotoxicity Targeted cancer therapies 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Laura V. Croft
    • 1
    Email author
  • Jaimie A. Mulders
    • 2
  • Derek J. Richard
    • 1
  • Kenneth O’Byrne
    • 1
    • 3
  1. 1.Faculty of Health, School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Cancer and Ageing Research Program, Translational Research InstituteQueensland University of TechnologyWoolloongabbaAustralia
  2. 2.TrendBio Pty Ltd.AlphingtonAustralia
  3. 3.Cancer ServicesPrincess Alexandra HospitalBrisbaneAustralia

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