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Cryodissection and Tissue Preparation of Drosophila Thorax for Indirect Flight Muscle Imaging

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Fundamental Approaches to Screen Abnormalities in Drosophila

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Abstract

Drosophila melanogaster has elaborate musculature comparable to the vertebrate specialized muscles, i.e. skeletal, cardiac and smooth. One of the largest fibrillar muscles found in the adult fly thorax is the indirect flight muscles (IFM). IFM is routinely used by the biologists to study muscle development, muscle attachment to the epidermis, cell biology, neuromuscular interaction, regulation of muscle contraction and signalling. This chapter gives an overview of the structure and development of IFM along with the procedure of dissection and imaging of IFM. This protocol can be used for morphological and phenotypic characterization of Drosophila muscle which will greatly help in our understanding of muscle biology pertaining to the myopathies and their aetiology.

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Acknowledgements

We acknowledge DBT-BUILDER Project (BT/PR-9028/INF/22/193/2013) for lab equipment to Department of Life Sciences, Central University of Jharkhand and Indian Council of Medical Research (ICMR) for Senior Research Fellowship (SRF) grant to M.D.

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Authors and Affiliations

  1. Department of Life Sciences, Central University of Jharkhand, CTI Campus, Ranchi, Jharkhand, India

    Madhavi Dubey, Kumari Pragati Nanda & Hena Firdaus

Authors
  1. Madhavi Dubey
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  2. Kumari Pragati Nanda
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  3. Hena Firdaus
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Corresponding author

Correspondence to Hena Firdaus .

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Editors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Monalisa Mishra

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Dubey, M., Nanda, K.P., Firdaus, H. (2020). Cryodissection and Tissue Preparation of Drosophila Thorax for Indirect Flight Muscle Imaging. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9756-5_6

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-9755-8

  • Online ISBN: 978-1-4939-9756-5

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