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Biochemical Assays to Detect the Antioxidant Level in Drosophila melanogaster

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Fundamental Approaches to Screen Abnormalities in Drosophila

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Abstract

Oxygen is a key molecule for the survivability of the aerobic animals, which is converted into toxic byproduct known as reactive oxygen species (ROS) under stress. ROS can induce damage to the cells. To protect the organism from ROS-induced damage, living organisms develop antioxidant defence system. The antioxidative enzymes include superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (GPx) and 1,1-diphenyl-2-picrylhydrazyl (DPPH). These antioxidative enzymes are also present in Drosophila to invade the ROS generated within the body. As a result, the antioxidant enzymes prevent disease development and increase the life span of the organism. Thus, indirectly the determination of antioxidant enzymes is a pointer of the health of the animal. The protocol describes various antioxidant assays in Drosophila melanogaster.

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Acknowledgements

JB is thankful to BT/PR21857/NNT/28/1238/2017 for financial support. MM lab is supported by Grant No. BT/PR21857/NNT/28/1238/2017, EMR/2017/003054 and Odisha DBT 3325/ST(BIO)-02/2017.

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Authors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Janmejaya Bag & Monalisa Mishra

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  1. Janmejaya Bag
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  2. Monalisa Mishra
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Correspondence to Monalisa Mishra .

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Editors and Affiliations

  1. Neural Developmental Biology Lab, Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Monalisa Mishra

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Bag, J., Mishra, M. (2020). Biochemical Assays to Detect the Antioxidant Level in Drosophila melanogaster. In: Mishra, M. (eds) Fundamental Approaches to Screen Abnormalities in Drosophila. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-9756-5_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9756-5_13

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-9755-8

  • Online ISBN: 978-1-4939-9756-5

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