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Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay

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Cryptosporidium

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2052))

Abstract

For more than 35 years, various assay formats have been used to detect Cryptosporidium-specific antibodies in human and animal sera. Cryptosporidium parvum 17- and 27-kDa antigens, identified from invasive sporozoites, have been used in serologic antibody assays to identify individuals infected in outbreaks of diarrheal disease caused by this protozoan parasite and to monitor exposures in communities. During infection, immunoglobulin (Ig) A, IgM, and IgG responses are elicited by these immunodominant antigens, and the parasite-specific Ig responses diminish following the resolution of infection. Using the recombinant forms of the 17- and 27-kDa C. parvum antigens and the relatively recently developed multiplex bead assay (MBA), data from serologic antibody responses can be economically and efficiently acquired, especially when the Cryptosporidium assays are integrated with assays for antibody responses to antigens from other pathogens monitored in community-wide or nation-wide serosurveys. Here we describe the coupling of the C. parvum recombinant antigens to carboxylated polystyrene beads, the data acquisition and analysis of IgG antibodies bound to the coupled beads, and the quality control methods required for data validation using the Luminex/MBA system.

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Acknowledgments

We would like to thank P.J. Lammie, S.E. Gwyn, G.C. Cooley, S. Dimitrova, and K. Robinson for helpful comments and suggestions on the manuscript.

Disclosures: Use of trade names is for identification only and does not imply endorsement by the Public Health Service or by the U.S. Department of Health and Human Services. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.

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Authors and Affiliations

  1. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

    Jeffrey W. Priest & Delynn M. Moss

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  1. Jeffrey W. Priest
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  2. Delynn M. Moss
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Correspondence to Jeffrey W. Priest .

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Editors and Affiliations

  1. Atlanta Veterans Affairs Medical Center, Decatur, GA, USA

    Jan R. Mead

  2. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

    Michael J. Arrowood

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Priest, J.W., Moss, D.M. (2020). Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay. In: Mead, J., Arrowood, M. (eds) Cryptosporidium. Methods in Molecular Biology, vol 2052. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9748-0_5

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