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Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation

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Electroporation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2050))

Abstract

Transdermal delivery of gene medicine holds a great promise in gene therapy, and electroporation-mediated method is a high-efficiency drug transfer technique. Traditional skin electroporation approaches require high voltage that may cause severe injure. In this chapter, we provide a detailed protocol of a novel skin electroporation approach via combination of a microneedle roller and a flexible interdigitated electroporation array (FIEA) for efficient delivery of DNA and siRNA into mouse skin. This electroporation protocol assisted by punching with the microneedle roller represents significant advantages over treatment with electroporation alone, allowing successful nucleic acid transportation at low voltage, with ideal safety outcomes. We describe the details of fabrication process of the FIEA, experiment preparation, and mouse thigh skin electroporation.

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Acknowledgments

This work was supported by the National Key Research and Development Program of China (No. 2016YFA0200802),

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Authors and Affiliations

  1. Advanced Research Institute of Multidisciplinary Science, and School of Life Science, Beijing Institute of Technology, Beijing, China

    Yuanyu Huang

  2. National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Institute of Microelectronics, Peking University, Beijing, China

    Dong Huang & Zhihong Li

Authors
  1. Dong Huang
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  2. Yuanyu Huang
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  3. Zhihong Li
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Corresponding author

Correspondence to Zhihong Li .

Editor information

Editors and Affiliations

  1. MD Anderson Cancer Center, The University of Texas, Houston, TX, USA

    Shulin Li

  2. School of Biological Science and Medical Engineering, Beihang University, Beijing, China, Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,, Beijing, China

    Lingqian Chang

  3. Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France

    Justin Teissie

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Huang, D., Huang, Y., Li, Z. (2020). Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation. In: Li, S., Chang, L., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9740-4_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9740-4_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9739-8

  • Online ISBN: 978-1-4939-9740-4

  • eBook Packages: Springer Protocols

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