Genome-Scale Metabolic Modeling from Yeast to Human Cell Models of Complex Diseases: Latest Advances and Challenges

  • Yu Chen
  • Gang Li
  • Jens NielsenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2049)


Genome-scale metabolic models (GEMs) are mathematical models that enable systematic analysis of metabolism. This modeling concept has been applied to study the metabolism of many organisms including the eukaryal model organism, the yeast Saccharomyces cerevisiae, that also serves as an important cell factory for production of fuels and chemicals. With the application of yeast GEMs, our knowledge of metabolism is increasing. Therefore, GEMs have also been used for modeling human cells to study metabolic diseases. Here we introduce the concept of GEMs and provide a protocol for reconstructing GEMs. Besides, we show the historic development of yeast GEMs and their applications. Also, we review human GEMs as well as their uses in the studies of complex diseases.

Key words

Biomarker Drug target Genome-scale metabolic models Human cells Metabolic engineering Saccharomyces cerevisiae Systems biology Yeast 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biology and Biological EngineeringChalmers University of TechnologyGothenburgSweden
  2. 2.Novo Nordisk Foundation Center for BiosustainabilityChalmers University of TechnologyGothenburgSweden
  3. 3.Novo Nordisk Foundation Center for BiosustainabilityTechnical University of DenmarkKongens LyngbyDenmark

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