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Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain

  • Karolina Miszczak
  • Boris EggerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2047)

Abstract

Live cell imaging gives valuable insights into the dynamic biological processes within and between cells. An important aspect of live cell imaging is to keep the cells under best physiological condition and to prevent abnormal cellular behavior, which might be caused by phototoxicity during microscopy. In this chapter we describe a protocol to visualize division patterns of neural stem cells in live whole mount brains of Drosophila larvae. We also present a newly developed live cell chamber that allows us to control the environmental air during live cell imaging. The protocol can be adapted to look at a wide range of cellular and tissue behavior in the Drosophila model system.

Keywords

Neural stem cells Drosophila Brain Live cell imaging 

Notes

Acknowledgments

We thank Jean-Daniel Niederhäuser for the construction of the live cell chamber and we thank Clemens Cabernard for advising us on live cell imaging protocols.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of BiologyUniversity of FribourgFribourgSwitzerland

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