Transplantation of Neural Tissue: Quail–Chick Chimeras
Tissue transplantation is an important approach in developmental neurobiology to determine cell fate, to uncover inductive interactions required for tissue specification and patterning as well as to establish tissue competence and commitment. Combined with state-of-the-art molecular approaches, transplantation assays have been instrumental for the discovery of gene regulatory networks controlling cell fate choices and how such networks change over time. Avian species are among the favorite model systems for these approaches because of their accessibility and relatively large size. Here we describe two culture techniques used to generate quail–chick chimeras at different embryonic stages and methods to distinguish graft and donor tissue.
KeywordsChick Brain Central nervous system Neural plate Neural tube Organizer Peripheral nervous system Quail Transplantation
This work is supported by the BBSRC, NIH, and ERC. We thank Anneliese Norris for assistance with New culture photography.
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