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Transplantation of Neural Tissue: Quail–Chick Chimeras

  • Andrea StreitEmail author
  • Claudio D. Stern
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2047)

Abstract

Tissue transplantation is an important approach in developmental neurobiology to determine cell fate, to uncover inductive interactions required for tissue specification and patterning as well as to establish tissue competence and commitment. Combined with state-of-the-art molecular approaches, transplantation assays have been instrumental for the discovery of gene regulatory networks controlling cell fate choices and how such networks change over time. Avian species are among the favorite model systems for these approaches because of their accessibility and relatively large size. Here we describe two culture techniques used to generate quail–chick chimeras at different embryonic stages and methods to distinguish graft and donor tissue.

Keywords

Chick Brain Central nervous system Neural plate Neural tube Organizer Peripheral nervous system Quail Transplantation 

Notes

Acknowledgments

This work is supported by the BBSRC, NIH, and ERC. We thank Anneliese Norris for assistance with New culture photography.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Faculty of Dental, Oral and Craniofacial Sciences, Centre for Craniofacial and Regenerative BiologyKing’s College LondonLondonUK
  2. 2.Department of Cell and Developmental BiologyUniversity College LondonLondonUK

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