Live Morphometric Classification of Sensory Neurons in Larval Zebrafish
Sensory systems convey environmental information to the brain. A comprehensive description of neuronal anatomy and connectivity is essential to understand how sensory information is acquired, transmitted, and processed. Here we describe a high-resolution live imaging technique to quantify the architecture of sensory neurons in larval zebrafish. This approach is ideal to assess neuronal-circuit plasticity and regeneration.
KeywordsSensory system Lateral line Zebrafish Neuronal morphometry
We thank the Central Animal Facility of the HMGU (Munich, Germany) for expert animal care. This work was supported by the Helmholtz Gemeinschaft.
Ethical considerations: All procedures on live specimens should be performed under guidelines and approved protocols by local and general/federal agencies. The method and experiments described here were performed according to EU Directive 2010/63/EU, under which an ethics committee-approved animal protocol is required for freely feeding zebrafish (older than 5.5 days). Work in our laboratory employs zebrafish of 5 days or younger.