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Live Morphometric Classification of Sensory Neurons in Larval Zebrafish

  • Gema Valera
  • Hernán López-SchierEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2047)

Abstract

Sensory systems convey environmental information to the brain. A comprehensive description of neuronal anatomy and connectivity is essential to understand how sensory information is acquired, transmitted, and processed. Here we describe a high-resolution live imaging technique to quantify the architecture of sensory neurons in larval zebrafish. This approach is ideal to assess neuronal-circuit plasticity and regeneration.

Keywords

Sensory system Lateral line Zebrafish Neuronal morphometry 

Notes

Acknowledgments

We thank the Central Animal Facility of the HMGU (Munich, Germany) for expert animal care. This work was supported by the Helmholtz Gemeinschaft.

Ethical considerations: All procedures on live specimens should be performed under guidelines and approved protocols by local and general/federal agencies. The method and experiments described here were performed according to EU Directive 2010/63/EU, under which an ethics committee-approved animal protocol is required for freely feeding zebrafish (older than 5.5 days). Work in our laboratory employs zebrafish of 5 days or younger.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Sensory Biology and OrganogenesisHelmholtz Zentrum MunichMunichGermany

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