CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila

  • Cornelia FritschEmail author
  • Simon G. Sprecher
Part of the Methods in Molecular Biology book series (MIMB, volume 2047)


Continuous implementation of new techniques allowing increasingly precise genetic manipulations makes the fruit fly Drosophila melanogaster an impacting model to study the nervous system. While transgenic approaches have been heavily used to investigate how the brain develops, genome editing has been notoriously hard in the fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene knockout.


Drosophila Brain Genome editing CRISPR Conditional alleles 



We thank S. Bullock, M. Harrison, K. O’Connor-Giles, and J. Wildoger for plasmids. Special thanks to Jenifer Kaldun for generating the pBsF3xHAF plasmid. We thank the Bloomington Drosophila Stock center for fly strains. This work was funded by the Swiss National Science Foundation (31003A_149499 to S.G.S.).


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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of BiologyUniversity of FribourgFribourgSwitzerland

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