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In Vitro Differentiation of T-Cells pp 131–141Cite as

In Vitro Differentiation of T Cells from Murine Pluripotent Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2048))

Abstract

In recent years cancer immunotherapy, especially the cell-based immunotherapy, has reached several milestones and achieved a lot of cancer remission in the clinics. Obtaining a more potent and effective cytotoxic T lymphocytes (CTLs) for cancer immunotherapy is always the ultimate goal for the researchers. However, the difficulty in harvesting a large number of tumor antigen-specific CTLs from the tumor patient is still a major obstacle we need to overcome. In our previous studies, it is shown that pluripotent stem cell-derived CTL—especially the genetically engineered antigen-specific CTLs—may serve as a good source of unlimited number of highly reactive and antigen-specific CTLs. Here we present a two-step method for the generation of antigen-specific T lymphocytes from iPS cells by in vitro priming and in vivo maturation.

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Acknowledgments

This project was funded, in part, under the grants from National Institute of Health Grant R01AI121180, R21AI109239, and R01CA221867 and the American Diabetes Association (1-16-IBS-281) to J.S.

Author information

Author notes

  1. Xiaoniao Chen and Fengyang Lei contributed equally to this work.

Authors and Affiliations

  1. Department of Ophthalmology, Chinese PLA General Hospital, Beijing, China

    Xiaoniao Chen & Liqiang Wang

  2. Department of Ophthalmology, Harvard Medical School, Boston, MA, USA

    Xiaoniao Chen & Fengyang Lei

  3. Department of Microbial Pathogenesis and Immunology, Texas A&M University, Bryan, TX, USA

    Xiaofang Xiong & Jianxun Song

Authors
  1. Xiaoniao Chen
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  2. Fengyang Lei
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  3. Liqiang Wang
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  4. Xiaofang Xiong
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  5. Jianxun Song
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Corresponding author

Correspondence to Jianxun Song .

Editor information

Editors and Affiliations

  1. Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Shin Kaneko

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Chen, X., Lei, F., Wang, L., Xiong, X., Song, J. (2019). In Vitro Differentiation of T Cells from Murine Pluripotent Stem Cells. In: Kaneko, S. (eds) In Vitro Differentiation of T-Cells. Methods in Molecular Biology, vol 2048. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9728-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9728-2_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9727-5

  • Online ISBN: 978-1-4939-9728-2

  • eBook Packages: Springer Protocols

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