Abstract
In many anaerobic environments methane (CH4) is produced by methanogens, with either H2/CO2 or acetate (i.e., the methyl group) as precursors, through what are referred to as hydrogenotrophic and acetoclastic methanogenic pathways respectively. Their relative contribution to total CH4 production can be quantified by determining the stable carbon isotopic fractionation factors for both pathways as well as the isotopic signatures of CO2, CH4, and the methyl group in acetate of the sample. The procedures for measuring carbon isotopic fractionation factors of both methanogenic pathways and isotopic composition of these compounds by isotope ratio mass spectrometry are described in this chapter. The results are very helpful in evaluating the activity of the methanogens involved in each methanogenic pathway as well as those of other biological pathways with different fractionation factors.
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Acknowledgments
This work was funded by the National Natural Science Foundation of China (41573083) and State Key Laboratory of Environmental Geochemistry (SKLEG2015403), China.
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Yuan, Q. (2019). Quantification of Methanogenic Pathways Using Stable Carbon Isotopic Signatures. In: Dumont, M., Hernández GarcÃa, M. (eds) Stable Isotope Probing. Methods in Molecular Biology, vol 2046. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9721-3_7
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DOI: https://doi.org/10.1007/978-1-4939-9721-3_7
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