Abstract
Metatranscriptomics is a powerful tool for capturing gene expression patterns in microbial communities and investigating their responses to environmental conditions. Stable isotope probing (SIP) is a method to target specific functional groups of microorganisms in environmental samples. The combination of RNA-SIP with metatranscriptomic analysis enhances the detection and identification of mRNA from target microorganisms. In this chapter we provide a protocol for RNA-SIP, mRNA enrichment, and mRNA preparation for high-throughput sequencing using an example of targeting methanotrophs in rice field soil.
Key words
- Metatranscriptome
- Stable isotope probing
- Soil microbiome
- 13C-mRNA
- High-throughput sequencing
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Hu, A., Lu, Y., Hernández García, M., Dumont, M.G. (2019). Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing. In: Dumont, M., Hernández García, M. (eds) Stable Isotope Probing. Methods in Molecular Biology, vol 2046. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9721-3_13
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DOI: https://doi.org/10.1007/978-1-4939-9721-3_13
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Publisher Name: Humana, New York, NY
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