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Flow Cytometry of Membrane Purinoreceptors

  • Nicole Schwarz
  • Marten Junge
  • Friedrich Haag
  • Friedrich Koch-NolteEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2041)

Abstract

Mammalian purinoreceptors respond to extracellular nucleotides and their metabolites, for example, following the release of ATP or NAD+ from cells and their hydrolysis by ectonucleotidases. Membrane purinoreceptors are expressed as ionotropic ligand-gated ion channels designated P2X receptors, or as metabotropic G-protein coupled receptors designated P1 or P2Y receptors, on the cell surface of different cell types. In this chapter, we provide protocols to monitor the expression and activity of purinoreceptors on the cell membrane of living cells by flow cytometry.

Key words

Purinoreceptors Purinergic signaling Flow cytometry Ca2+-influx Externalization of phosphatidylserine Ectodomain shedding Inflammasome P2X7 

Notes

Acknowledgments

The authors would like to acknowledge the EU COST Program (BM1406).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Nicole Schwarz
    • 1
  • Marten Junge
    • 1
  • Friedrich Haag
    • 1
  • Friedrich Koch-Nolte
    • 1
    Email author
  1. 1.Institute of ImmunologyUniversity Medical Center Hamburg-EppendorfHamburgGermany

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