Assessment of Cell Adhesion After Purinoceptor Activation
Cell adhesion is a characteristic feature of phagocytic myeloid cells, which is important for several inflammatory processes, such as migration, invasion, and proliferation. Purinergic signaling in macrophages plays an important role in cell adhesion of this cell type to different extracellular substrates. This protocol describes the use of two different detection methods to quantify cell adhesion upon P2X7 receptor activation by extracellular ATP.
Key wordsCell adhesion P2X7 receptor Macrophage Extracellular matrix Extracellular ATP MTT Yo-Pro-1
This work was supported by the European Research Council (ERC-2013-CoG 614578), EU COST Program (BM1406), and Ministerio de Economia, Industria y Competitividad–Fondo Europeo de Desarrollo Regional (project no. SAF2017-88276-R).
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