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Assessment of Cell Adhesion After Purinoceptor Activation

  • Juan Jose Martínez-García
  • Pablo PelegrínEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2041)

Abstract

Cell adhesion is a characteristic feature of phagocytic myeloid cells, which is important for several inflammatory processes, such as migration, invasion, and proliferation. Purinergic signaling in macrophages plays an important role in cell adhesion of this cell type to different extracellular substrates. This protocol describes the use of two different detection methods to quantify cell adhesion upon P2X7 receptor activation by extracellular ATP.

Key words

Cell adhesion P2X7 receptor Macrophage Extracellular matrix Extracellular ATP MTT Yo-Pro-1 

Notes

Acknowledgments

This work was supported by the European Research Council (ERC-2013-CoG 614578), EU COST Program (BM1406), and Ministerio de Economia, Industria y Competitividad–Fondo Europeo de Desarrollo Regional (project no. SAF2017-88276-R).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Biomedical Research Institute of Murcia (IMIB)Clinical University Hospital Virgen de la ArrixacaMurciaSpain

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