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Recording P2X Receptors Using Whole-Cell Patch Clamp from Native Monocytes and Macrophages

  • Leanne StokesEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2041)

Abstract

Investigating ion channels in their native cell type is important when striving to understand their regulation and function, but this comes with added complexities due to the plethora of channels and receptors present. Details of recording ATP-gated ion channels in macrophages are presented together with information on how to prepare the primary cells for electrophysiological analysis.

Key words

Whole-cell patch clamp P2X receptor Macrophage ATP EPC10 P2X7 P2X4 Monocyte 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.School of PharmacyUniversity of East AngliaNorwichUK

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