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P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes

  • Eléonore Bertin
  • Audrey Martínez
  • Eric Boué-GrabotEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2041)

Abstract

Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.

Key words

P2X ATP-gated channels Double-electrode patch clamp Electrophysiology Receptor function Surface trafficking Xenopus Oocytes 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Eléonore Bertin
    • 1
  • Audrey Martínez
    • 1
  • Eric Boué-Grabot
    • 1
    Email author
  1. 1.Institut des Maladies Neurodégénératives, CNRS UMR 5293Université de BordeauxBordeauxFrance

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