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Multimeric Purinoceptor Detection by Bioluminescence Resonance Energy Transfer

  • Vincent CompanEmail author
  • François Rassendren
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2041)

Abstract

Assays based on bioluminescence resonance energy transfer (BRET) provide a sensitive and simple method to study protein–protein interactions in live cells. Here we describe a protocol using BRET technique to investigate potential interactions between P2X subunits. This approach combined with bimolecular fluorescence complementation (BiFC) can also be employed to determine the stoichiometry of heteromeric P2X receptors.

Key words

Bioluminescence BRET P2X BiFC Stoichiometry Heteromer 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.IGF, University of Montpellier, CNRS, INSERMMontpellierFrance
  2. 2.Labex ICSTMontpellierFrance

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