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Multimeric Ionotropic Purinoceptor Detection by Protein Cross-Linking

  • Vincent CompanEmail author
  • François Rassendren
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2041)

Abstract

P2X receptor subunits (P2X1 to P2X7) assemble to form trimeric homomers or heteromers. Here, we describe the use of protein cross-linking to study the composition of P2X receptor complexes. This simple protocol is useful for determining the stoichiometry of P2X heteromeric receptors as well as for assessing the effect of point mutation, truncation, or concatenation on the quaternary architecture of these receptors.

Key words

P2X Cross-linking Heteromer Homomer Trimer Quaternary structure 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.IGF, University of Montpellier, CNRS, INSERMMontpellierFrance
  2. 2.Labex ICSTMontpellierFrance

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