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Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils

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ADAMTS Proteases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2043))

Abstract

The extracellular matrix (ECM) is a composite biomaterial that serves as an anchor for cells and provides guidance cues for cell migration, proliferation, and differentiation. However, many details of the hierarchical ECM assembly process and the role of individual protein–protein interactions are not well understood. Here, I describe a cell-culture-based method that allows for determination of the ECM localization of recombinant ADAMTS proteases and ADAMTS-like (L) proteins in relationship to fibrillin microfibrils deposited by human dermal fibroblasts. The method can be readily adapted to study the localization of ECM components other than ADAMTS and ADAMTSL proteins to fibrillin microfibrils and other ECM networks.

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Acknowledgments

This work was supported by NIH grant AR070748.

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Authors and Affiliations

  1. Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Dirk Hubmacher

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  1. Dirk Hubmacher
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Correspondence to Dirk Hubmacher .

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Editors and Affiliations

  1. Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA

    Suneel S. Apte

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Hubmacher, D. (2020). Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils. In: Apte, S. (eds) ADAMTS Proteases. Methods in Molecular Biology, vol 2043. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9698-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9698-8_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9697-1

  • Online ISBN: 978-1-4939-9698-8

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