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Purification of Recombinant ADAMTSL2

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ADAMTS Proteases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2043))

Abstract

Recombinantly produced proteins are used in many biological disciplines. However, their purity and quality are vital for downstream applications used to determine their structure and functions. Several purification and detection strategies can be used in combination to obtain protein samples with homogeneity and structural conformity. Here we detail the protocols involved in the purification of ADAMTSL2 from mammalian cells. We also describe the protocols used to validate the purity of the protein samples.

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Acknowledgments

MS was supported by a Medical Research Council (MRC) grant (ref: MR/L016540/1) and a Biotechnology and Biological Sciences Research Council (BBSRC) studentship to CB. The Wellcome Centre for Cell-Matrix Research is supported by core grant funding from the Wellcome Trust (088785/Z/09/Z).

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Authors and Affiliations

  1. Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, Wellcome Centre for Cell-Matrix Research, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK

    Mukti Singh & Clair Baldock

Authors
  1. Mukti Singh
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  2. Clair Baldock
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Corresponding author

Correspondence to Clair Baldock .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA

    Suneel S. Apte

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Singh, M., Baldock, C. (2020). Purification of Recombinant ADAMTSL2. In: Apte, S. (eds) ADAMTS Proteases. Methods in Molecular Biology, vol 2043. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9698-8_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9698-8_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9697-1

  • Online ISBN: 978-1-4939-9698-8

  • eBook Packages: Springer Protocols

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