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Fast NMR Methods for Identification of Resonances and Metabolic Pathways

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 2037)

Abstract

High-throughput analysis of NMR data in metabolomics involves both rapid data acquisition and analysis. We describe here a data collection and analysis protocol, which enables fast multidimensional NMR data acquisition and automated analysis of NMR spectra to rapidly identify the metabolites and assign them to active metabolic pathways in the system.

Key words

Fast NMR methods Multiple receivers Pattern recognition Metabolite assignments Metabolic pathways 
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Notes

Acknowledgments

The high-field NMR facility at NMR Research Centre, Indian Institute of Science, supported by the Department of Science and Technology (India) is gratefully acknowledged. HSA acknowledges research grant from SERB (EMR/2016/003181).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.NMR Research CentreIndian Institute of ScienceBangaloreIndia
  2. 2.Department of Biochemistry and Molecular Pharmacology, Dana-Farber Cancer InstituteHarvard Medical SchoolBostonUSA
  3. 3.Department of Cancer Systems ImagingUniversity of Texas MD Anderson Cancer CenterHoustonUSA

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