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Lipid Profiling Using 1H NMR Spectroscopy

Part of the Methods in Molecular Biology book series (MIMB,volume 2037)

Abstract

Lipid profiling, which includes fatty acids, phospholipids, glycerides, and cholesterols is extremely important because of the essential role lipids play in the regulation of metabolism in animals. 1H-NMR-based protocols for high-throughput lipid analysis in complex mixtures have been developed and applied to biological systems. Many classes of lipids can be quantitatively analyzed in many sample matrices including serum, cells, and tissues using a simple 1H NMR experiment. In this chapter, we provide protocols for NMR-based lipid profiling including sample preparation, NMR experiments, and quantification using the LipSpin software tool.

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Acknowledgment

The authors wish to acknowledge the financial support of the Spanish Ministry of Economy and Competitiveness through project TEC2015-69076-P.

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Correspondence to Xavier Correig .

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Gil, M., Samino, S., Barrilero, R., Correig, X. (2019). Lipid Profiling Using 1H NMR Spectroscopy. In: Gowda, G., Raftery, D. (eds) NMR-Based Metabolomics. Methods in Molecular Biology, vol 2037. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9690-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9690-2_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9689-6

  • Online ISBN: 978-1-4939-9690-2

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