Abstract
Lipid profiling, which includes fatty acids, phospholipids, glycerides, and cholesterols is extremely important because of the essential role lipids play in the regulation of metabolism in animals. 1H-NMR-based protocols for high-throughput lipid analysis in complex mixtures have been developed and applied to biological systems. Many classes of lipids can be quantitatively analyzed in many sample matrices including serum, cells, and tissues using a simple 1H NMR experiment. In this chapter, we provide protocols for NMR-based lipid profiling including sample preparation, NMR experiments, and quantification using the LipSpin software tool.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Watson AD (2006) Thematic review series: systems biology approaches to metabolic and cardiovascular disorders. Lipidomics: a global approach to lipid analysis in biological systems. J Lipid Res 47:2101–2111
Quehenberger O, Armando AM, Brown AH, Milne SB, Myers DS, Merrill AH et al (2010) Lipidomics reveals a remarkable diversity of lipids in human plasma. J Lipid Res 51:3299–3305
German JB, Gillies LA, Smilowitz JT, Zivkovic AM, Watkins M (2007) Lipidomics and lipid profiling in metabolomics. Curr Opin Lipidol 18:66–71
Murphy SA, Nicolaou A (2013) Lipidomics applications in health, disease and nutrition research. Mol Nutr Food Res 57:1336–1346
Soininen P, Kangas AJ, Würtz P, Suna T, Ala-Korpela M (2015) Quantitative serum nuclear magnetic resonance metabolomics in cardiovascular epidemiology and genetics. Circ Cardiovasc Genet 8:192–206
Miao H, Chen H, Pei S, Bai X, Vaziri ND, Zhao YY (2015) Plasma lipidomics reveal profound perturbation of glycerophospholipids, fatty acids, and sphingolipids in diet-induced hyperlipidemia. Chem Biol Interact 228:79–87
Goto-Inoue N, Yamada K, Inagaki A, Furuichi Y, Ogino S, Manabe Y et al (2013) Lipidomics analysis revealed the phospholipid compositional changes in muscle by chronic exercise and high-fat diet. Sci Rep 3:3267. https://doi.org/10.1038/srep03267
Meikle PJ, Wong G, Barlow CK, Kingwell BA (2014) Lipidomics: potential role in risk prediction and therapeutic monitoring for diabetes and cardiovascular disease. Pharmacol Ther 143:12–23
Sethi S, Brietzke E (2017) Recent advances in lipidomics: analytical and clinical perspectives. Prostaglandins Other Lipid Mediat 128–129:8–16
Soininen P, Kangas AJ, Würtz P, Tukiainen T, Tynkkynen T, Laatikainen R et al (2009) High-throughput serum NMR metabonomics for cost-effective holistic studies on systemic metabolism. Analyst 134:1781. https://doi.org/10.1039/b910205a
Mallol R, Amigó N, Rodríguez MA, Heras M, Vinaixa M, Plana N et al (2015) Liposcale: a novel advanced lipoprotein test based on 2D diffusion-ordered 1H NMR spectroscopy. J Lipid Res 56:737–746
Silbernagel G, Pagel P, Pfahlert V, Genser B, Scharnagl H, Kleber ME et al (2017) High-density lipoprotein subclasses, coronary artery disease, and cardiovascular mortality. Clin Chem 63:1886–1896
Jeyarajah J, Cromwell EJ, Otvos WC (1981) Lipoprotein particle analysis by nuclear magnetic resonance spectroscopy. Clin Lab Med 26:847–855
Vinaixa M, Ángel Rodríguez M, Rull A, BeltrÁn R, Bladé C, Brezmes J et al (2010) Metabolomic assessment of the effect of dietary cholesterol in the progressive development of fatty liver disease. J Proteome Res 9:2527–2538
Folch J, Lees M, Stanley G (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226:497–509
Löfgren L, Ståhlman M, Forsberg GB, Saarinen S, Nilsson R, Hansson GI (2012) The BUME method: a novel automated chloroform-free 96-well total lipid extraction method for blood plasma. J Lipid Res 53:1690–1700
Barrilero R, Gil M, Amigó N, Dias CB, Wood LG, Garg ML et al (2018) LipSpin: a new bioinformatics tool for quantitative 1H NMR lipid profiling. Anal Chem 90:2031–2040
Chen L, Weng Z, Goh L, Garland M (2002) An efficient algorithm for automatic phase correction of NMR spectra based on entropy minimization. J Magn Reson 158:164–168
Oostendorp M (2006) Diagnosing inborn errors of lipid metabolism with proton nuclear magnetic resonance spectroscopy. Clin Chem 52:1395–1405
Bharti SK, Roy R (2012) Quantitative 1H NMR spectroscopy. TrAC Trends Anal Chem 35:5–26
Serkova N, Florian Fuller T, Klawitter J, Freise CE, Niemann CU (2005) 1H-NMR–based metabolic signatures of mild and severe ischemia/reperfusion injury in rat kidney transplants. Kidney Int 67:1142–1151
Acknowledgment
The authors wish to acknowledge the financial support of the Spanish Ministry of Economy and Competitiveness through project TEC2015-69076-P.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Gil, M., Samino, S., Barrilero, R., Correig, X. (2019). Lipid Profiling Using 1H NMR Spectroscopy. In: Gowda, G., Raftery, D. (eds) NMR-Based Metabolomics. Methods in Molecular Biology, vol 2037. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9690-2_3
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9690-2_3
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9689-6
Online ISBN: 978-1-4939-9690-2
eBook Packages: Springer Protocols