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Fast Quantitative 2D NMR for Untargeted and Targeted Metabolomics

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 2037)

Abstract

Nuclear magnetic resonance (NMR) spectroscopy exhibits a great potential for the quantitative analysis of complex biological samples such as those encountered in metabolomics. To overcome the ubiquitous problem of overlapping peaks in 1D NMR spectra of complex mixtures, acquisition of 2D NMR spectra allows a better separation between overlapped resonances while yielding accurate quantitative data when appropriate analytical protocols are implemented. The experiment duration can be made compatible with high-throughput studies on large sample collections by relying on fast acquisition methods. Here, we describe the general metabolomics workflow to acquire fast quantitative 2D NMR data with a focus on targeted or untargeted analyses.

Key words

2D NMR Quantitative analysis Metabolomics Targeted Untargeted Fast methods 
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Notes

Acknowledgements

The authors acknowledge all the members of the EBSI team as well as external collaborators for fruitful discussions. Support from the CORSAIRE metabolomics platform (Biogenouest network) is also acknowledged.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.CEISAM, Université de Nantes, CNRS, UMR 6230NantesFrance
  2. 2.SpectroMaitrise, CAPACITES SASNantesFrance
  3. 3.Institut Universitaire de FranceParisFrance

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