Abstract
Proton (1H) nuclear magnetic resonance (NMR) spectroscopy can measure a range of metabolites in both cardiac tissue and blood plasma for following cardiovascular disease. For solution-state NMR spectroscopy, it is necessary to create a tissue extract, with perchloric acid, acetonitrile/water, and chloroform/methanol being popular extraction media. Alternatively, high-resolution magic angle spinning (HRMAS) 1H NMR spectroscopy can be used to derive a metabolic profile directly from intact cardiac tissue. This chapter will discuss the practical methods used for 1H NMR spectroscopy to follow cardiovascular diseases both in terms of metabolic changes in cardiac tissue and changes in blood plasma.
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Acknowledgments
This work was funded by the Medical Research Council (Lipid Profiling and Signaling, MC UP A90 1006, and Lipid Dynamics and Regulation, MC PC 13030).
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Griffin, J.L., Castro, C. (2019). NMR-Based Metabolomics in Cardiac Research. In: Gowda, G., Raftery, D. (eds) NMR-Based Metabolomics. Methods in Molecular Biology, vol 2037. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9690-2_11
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DOI: https://doi.org/10.1007/978-1-4939-9690-2_11
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